TESTS FOR VITAMIN C
Numerous methods for the detection and estimation of Vitamin C are available, but only those simple tests suitable for immediate use in the doctor’s surgery, in a clinic or to test foods at home or where they are prepared will be described.
1. The Dip Stick is perhaps the simplest. Ames C-Stix are available from any chemist shop. The slip of medicated paper is dipped into a specimen of urine and after 30 seconds the color compared with a chart that shows the comparative concentration in the urine. To obtain a true estimation it is important that the specimen be tested as early as possible, as Vitamin C in urine is quickly oxidized and destroyed on exposure to air. Dr Glen Dettman of Oakleigh Laboratories, Mentone, Victoria, who has tested routinely over 6000 urine specimens in the last 3 years has found that if a random urine specimen does not exceed the top color marker (40 mg%) there is either insufficient ascorbic acid in the body or the kidneys are not working well. Ames C-Stix can also be used to detect Vitamin C in foods and other fluids.
2. Mercks Pharmaceutical firm also make a similar dip stick, but this is not suitable for screening urine (so says their brochure), but rather for estimating C in food products, such as orange cordial and mixed dishes.
3. Lingual or tongue tests are used by Professors Cheraskin and Ringsdorf in their laboratories at the University of Alabama, and by Dr Archie Kalokorinos at the Redfern Clinic in Sydney. The test is simple and quick.
The tongue is cleaned and wiped dry and a small piece of paper infiltrated with a special indicator (Tillmans) is pressed against the tongue. The time taken in seconds for the blue color of the reagent to disappear after it touches the tongue indicates the ascorbic acid level in the body.
Optimum values — less than 20 seconds show normal Vitamin C status. Longer than 20 seconds indicates poor tissue ascorbic acid level. Each dye vial is sufficient for 70 individual tests and lasts 4 days after the solution is made.
A complete ascorbic acid testing kit can be obtained by writing to —
Medical Diagnostic Services Inc., P.O. Box 144,
BRANDON, FLORIDA. U.S.A. 33511.
Hospital wards might therefore not only test the urine of their patients if desired, but also Vitamin C intake including fluids such as synthetic orange juice, tomato juice and carrot juice. Infants Vitamin C ‘supplement’ may be readily oxidized if left for any time in our drinking water.
The following is a list of average values of Vitamin C in various foods tested with Ames C-Stix quoted by Drs Glen Dettman and Archie Kalokerinos in their paper to a Wollongong Conference of AIMT and ACBS April,
Values of Food estimated with C-Stix. (All in mg%) Where necessary juice was expressed from food to obtain results.
1973.
Typical Average Values (After Sebell/Harris)
Apples (temperature controlled)
Apple (old season, stored)
Apple (new season)
Canteloupe
Carrot (stored)
Cucumber
Cabbage
Celery
Green Peppers Grapes (small variety)
*13/21/7*
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